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KMID : 0364820220580020099
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Korean Journal of Microbiology
2022 Volume.58 No. 2 p.99 ~ p.101
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Draft genome sequence of Rhizopus delemar SSU VMBB-02 isolated from alcohol fermentation starter culture
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Lim Jae-Yun
Seo Jeong-Ah
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Abstract
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Filamentous fungi and yeasts were isolated from banh men, a fermentation starter culture for the production of traditional liquor in Vietnam. The genus Rhizopus was a major group of fungi, including Rhizopus oryzae, Rhizopus delemar, and Rhizopus microsporus isolated from banh men. Among twenty-five Rhizopus strains isolated, R. delemar SSU VMBB-02 (KCTC 46675) strain that highly produces hydrolytic enzymes, was selected for constructing the draft genome. The whole genome was sequenced, assembled and analyzed using Illumina HiSeq platform technology. As a result of genome analysis, the draft genome was assembled with the size of 37.9 Mb containing 34.4% G + C content and 12,076 protein-coding genes. Through additional analyses, the number of genes encoding carbohydrate- active enzymes and proteolytic enzymes, and the number of secondary metabolite biosynthesis gene clusters were revealed. The results of this study will provide useful genomic information that can be compared with other Rhizopus species originated from fermentation starter culture.
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KEYWORD
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Rhizopus delemar, draft genome sequence, fermentation starter culture, next generation sequencing technology
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